The goals of this Core Facility are to provide flow cytometry services for Howard University’s research investigators, and develop new applications.
"The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system. Designed with multicolor application support in mind, the BD FACSCalibur flow cytometer provides flexibility to support a wide variety of research and clinical applications."
Analysis of user-prepared samples by staff and sorting (isolation) of sub-populations of cells.
"Consultation in Assay Design. A consultation is required before initiating any new projects."
Graphical and statistical analysis of flow cytometry data.
Provides training on the FACSCalibur cell sorter and related software.
Provides access to flow cytometry equipment.
"BD CellQuest Pro software allows you to acquire and analyze data from your flow cytometer on a Macintosh® computer. Working in the BD CellQuest Pro Experiment document window, you can create several types of plots, including multicolor contour plots and overlaid histograms, and you can generate statistics for dot plots, histograms, density plots, 3D plots, and contour plots. The versatile graphics tool palette helps you produce presentation-quality documents. You can also save everything in an Experiment document, including plots, regions, gates, markers, statistics, calculated expressions, Browser contents, annotations, and color preferences, and then restore it all later."
"Trained on thousands of sample files, ModFit LT features a robust automatic analysis engine. With sophisticated peak detection, the system identifies ploidy patterns and dynamically creates models to match. Improvements like AutoDebris™, AutoAggregate™, and AutoLinearity™ make analysis more robust and consistent from one user to the next."