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Center for Sickle Cell Disease

Summary:

Howard University's Center for Sickle Cell Disease (SCD) was founded by the late Dr. Roland B. Scott in 1971 to address the needs of patients and families in the Washington Metropolitan area affected by SCD. The Center is committed to a six-fold goal that includes comprehensive medical care, research, testing, education, counseling, and community outreach. Recently, the Center has expanded its clinical research program and developed a collaborative consortium with Children’s National Medical Center (CNMC) and in working together with Howard University Hospital and NIH we are the Washington area’s leading provider of patient services for SCD.

Affiliations:

People:

      Member: Nekhai, Sergei, Ph.D.
      Role: Assistant professor of biochemistry, College of Medicine, Director, RCMI Proteomics Core Facility, Research scientist, Center for Sickle Cell Disease

      Member: Niu, Xiaomei, MD
      Role: Research Scientist

    Resources:

    Instruments

    • Bio-Rad Bio-Plex suspension array system ( Bead array reader )

      "The Bio-Plex Suspension Array System is a flexible, easy-to-use multiplex analysis system based on Luminex xMAP® microspheres and flow cytometry array technology that allows the simultaneous analysis of up to 100 different bio-molecules (proteins, peptides, or nucleic acids) in a single microplate well.

      Dramatically increase the amount of useful data from rare or volume-limited samples such as mouse or rat serum, and decipher complex interrelationships among proteins involved in, for example, cell signalling and transduction pathways.

      Major applications include cytokine profiling, phosphoprotein detection, isotyping and measurement of biomarkers in disease conditions such as diabetes, acute phase response and angiogenesis. "

    • Trinity Biotech Ultra2- variant resolution system ( High performance liquid chromatography instrument )

      "Trinity Biotech’s ultra2 Resolution system for the separation and quantitation of normal and variant hemoglobin species in blood is designed to provide the maximum amount of information in the shortest possible time. This method combines the sensitivity and specificity of HPLC with sample preparation automation and assistance in evaluating the result."

      "Specifications:
      Correlated to column chromatography for A2 quantitation
      Correlated to alkali denaturation for F quantitation
      Provides a screening and confirmatory testing
      Extensive variant library: detection of over 200 variants
      Variety of sample formats concurrently:
      Whole blood
      Haemolysates
      Dried blood spots for neonatal / newborn testing
      Large sample capacity:
      Tubes
      Vial
      Microtiter plates

      Barcode reading – positive sample ID from specimen to end result
      Automated – walk-away, cost-effective operation
      Selectable Test Methods:

      Quick Scan (screening) followed by High Resolution(confirmatory)
      Quick Scan only
      High Resolution only

      Relative retention times: 0.001 minutes
      Hb measurement starts at time zero:

      No calibration for A2 and F quantitation necessary

      Superior performance:

      Sensitivity of 0.1%, Principle Ion-exchange high performance liquid chromatography "


    Web Links:

    Last updated: 2012-09-07T13:57:35.956-05:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016